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The protein interaction network of the known epithelial growth factors in the posterior intestine were extracted from STRING v10 [ 34 ] using human as model organism as the database coverage is the highest. A protein interaction networks among genes that expression was upregulated, downregulated, unchanged were constructed by overlapping known interacting partner genes extracted from the database [ 26 ].

Plasma Na concentration, tissue Na content, moisture, and Na concentration, gene expressions in at various times following FW to SW transfer were analyzed by one-way ANOVA followed by Tukey’s multiple-comparison test (GraphPad Prism Ver. 6.0 for Windows, CA, USA). Bound Na contents in the luminal secretions of esophagus and intestine (FW vs. SW 3h), gene expression between FW to FW (control, 1h, and 3h) vs. FW to SW (salinity, 1h, and 3h) in gill, anterior and posterior intestine of eels were analyzed by two-way ANOVA, Bonferroni’s test. <0.05 is considered as significant different among comparisons.

Eel plasma Na + did not significantly increase after 3h (8.9% increase from FW), gradually reached statistical significant after 1 d (24.5% increase from FW), and subsequently established to a lower baseline after 7 d in SW (Fig. 1a ). Plasma Na + in medaka significantly increased by 21.1% from 147±2.0mM to 178±4.8mM (p<0.05) 3h after transfer from FW to 2/3 SW (Table 1 ) and returned to a new baseline (166±1.6mM) after 1 d.

Na-K-2Cl cotransporter 1α (NKCC1α, SLC12A2 a) in the gill was gradually upregulated and became significant after 7 d in SW (Fig. Tamaris Black Women’s Oxfords Schwarz Black 001 OSIjltjerP
). Gill specific CFTR b expression was upregulated in a similar delayed fashion as of NKCC1α (Fig. 1c ). Na-K-2Cl cotransporter 2β (NKCC2β, SLC12A1 b) was expressed most abundantly in the anterior and middle intestine among the digestive tract and the upregulation became significant in anterior intestine after 12h in SW but not in middle intestine. The upregulation reached maximum in 1 to 3 d, and returned to a lower level after 7 d in SW (Fig. 1d ).

The immunohistochemistry results of CFTR and NKCC were similar to those of gene expression. Tissue sections from different salinity treatment were mounted on the same slides to ensure identical antibody incubation and color development for comparison. Apical CFTR signals on the ionocytes were observed in after 7 d acclimation in SW (Fig. 2f , box), but were not apparent in the gill of early SW transferred eels (Fig. New Balance 520 Trainers Pink Pink/White 8lnXj
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). Basolateral NKCC staining was observed scarcely in the ionocytes in eels from FW to SW 1 d, while increasing intensity and density of NKCC positive ionocytes were observed in SW 3 d and 7 d individuals (Fig. Muium Women Ladies Open Toe Flat Wedge Sandals Ankle Buckle Rome Slippers Shoes White L9SF0xf
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). In the anterior intestine, NKCC signals were found on the apical membrane of the mucosal epithelia in all individuals, with an increasing intensity from SW 3 d to SW 7 d (Fig. Odomolor Womens PU Buckle RoundToe KittenHeels Solid PumpsShoes Gray 40 ntSmTwfA
- r ). No apparent localization changes were observed among branchial and intestinal CFTR and NKCC during early SW exposure (SW 3h to 12h).

Then you have a company like Ben Jerry’s, whose content seeks to promote company values. Yes, you’ll find a lot of their posts revolve around ice cream recipes and company trivia, but they also publish a large volume of content that speaks to a larger brand mission of improving the world, such as “ Nike Women’s 807313003 Fitness Shoes Black 3 LlBAtIf
” and “ 5 Travel Innovations to Help Save Our Climate .”

Jay Curley, senior global marketing manager at Ben Jerry’s, writes :

“We’re trying to create a new model for how businesses can use their voice to have an impact on important social movements, and show that you can do that and it doesn’t hurt your business. As a matter of fact, it may help.”

No matter what your campaign goals are, the content should align with your brand and message. It’s just a matter of how explicit you decide to be.

For many companies, it’s difficult to attract customers to your product and increase awareness about your brand without actually talking about it.

One way to still mention the brand without it being the main focus of the work is to craft your story not on the product itself, but around the experience that the product provides.

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, the car company keeps its brand in the background and places the focus on the people behind the driver’s wheel. The publication tells stories about couples and families on road trips or taking up challenges, instead of talking directly about its vehicles.

This goes to show that even the product-centric car industry can get creative with its content. The benefit of the product is still central, but the focus is on experience over objects.

As a UK department store selling a slew of products, John Lewis is another company that has mastered the approach of conspicuously incorporating a ton of products into its storytelling. John Lewis nails the balance with its Christmas campaigns year after year, including its beloved Buster the Boxer campaign featuring the adorable trampoline adventures of a dog, two foxes, and a hedgehog! It’s estimated that the brand’s sales have Dunlop Womens Zelda Sling Back Toe Thong Diamante Wedge Sandal Lapis Blue xGcDRl
due to the success and memorable storytelling used throughout these ads.

As we saw with Ben Jerry’s earlier, it’s difficult for food companies not to focus on their brand. Even so, there doesn’t always need to be a product focus. Much of BOSS Green Men’s ShuttleTennTech LowTop Sneakers Black I8APC0Cx
is heavy on brand mentions but topic categories include things like values, culture, inspiration, and nutrition, with very little about the product itself.

by Allhqfashion Womens Round Closed Toe Kitten Heels Soft Material Low top Solid Boots Black 2In9NRWu

Daniel Tay is a content marketer and writer. As the founder of With Content, he helps companies to create quality content that resonates with their audience of choice. With over 5 years of experience in content strategy and marketing, his writing can be found on websites such as Hubspot, Search Engine Journal, and Tech in Asia. You can find Daniel on LinkedIn or Twitter.

See more from Daniel

Although it seems counter-intuitive, less can certainly be more when comes to talking about your brand within your storytelling.

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Managing priorities

Tracker lists stories in a project’s Current and Backlog panels in priority order. Dragging a story to the top of your Backlog makes it the top priority (after the stories already in the Current iteration). You can change the priority of a story in the Current panel that is not yet accepted by dragging it within the Current panel. You can also change the priority of a story using the keyboard . Unstarted stories cannot be dragged above started stories, as it’s assumed that the most important stories are already being worked on. Drag stories from the Icebox to the desired location in the Backlog to prioritize them.

Accepted stories are displayed according to the date they were accepted. If you need to change the order of an accepted story, you can expand the story and click on the calendar icon to change the accepted date. Also, accepted stories remain in the iteration they were accepted in, and only move to the Done panel when that iteration is complete.

You can choose from a variety of ways to make priorities visible in Tracker. If you need to move many stories around in your Backlog or Icebox, cloning the panel to more easily drag them to a new position helps. Selecting multiple stories for dragging to a new location, or for updating via the bulk actions menu, can also save you time.

Stories within an Epic Stories panel are also listed in priority order. You can prioritize stories within an Zaza LifeSucksBoySloganPrintedUnisexFlipFlopsSmall Sat9KVPjK
by dragging them directly within the Epic Stories panel, up into the Backlog section or down into the Icebox section. When you add a story to an epic by dragging it to the Epic Stories panel, you can drop it in the location you want to reflect its priority.

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to group related stories and clearly reflect their priority.

Note :

Tracker automatically moves stories from the top of your project’s Backlog into the current iteration according to the current velocity . Although you won’t be able to drag and drop more stories into Current (you cannot drag more story points into Current that your velocity is currently worth), you can click Start on any story in the Backlog and it will automatically move to Current.

As your iterations progress and your stories move from the Backlog to Current , you will see the planned iterations in your Backlog respond accordingly based on the total points planned compared to your current velocity. For example, if you have 40 points’ worth of stories planned in your Backlog and your project velocity is currently 8, Tracker will plan these out over five iterations of about 8 points each (depending on the individual story estimates ). As you drag estimated stories to change priority order within the backlog, Tracker will update which stories are in each iteration to reflect the current velocity. Tracker will create new iterations as needed to accommodate newly estimated stories or updates to current story estimates. Going back to our example where your project velocity is 8, if you drag a 5-point story from the Icebox to the top of your Backlog, Tracker will move the stories that no longer fit in that iteration down to the next one, and reorganize future iterations accordingly.

When you are ready to find out what Wildfire can do for your business, we’ll be ready to show you.

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